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AAT Bioquest

FAQ

Posted on November 17, 2023
What are the principles of the TUNEL assay?
Apoptosis and NecrosisCaspasesDNA and RNADNA FragmentationDNA & RNA Markers and Ladders


Posted on September 7, 2023
How is proteinase K involved in cell lysis?
Antibodies and ProteomicsDNA and RNAEnzymesNucleic AcidsProteincell lysis


Posted on September 7, 2023
Is there an alternative to using proteinase K during DNA extractions?
Antibodies and ProteomicsDNA and RNADNA extractionEnzymesProtein


Posted on August 5, 2023
How are recombinant proteins made?
Antibodies and Proteomics BioconjugationDNA and RNAPolymerase Chain Reaction (PCR)ProteinTransfection


Posted on August 5, 2023
What are recombinant proteins used for?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the examples of recombinant proteins?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
How does recombinant protein expression work?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
Is insulin a recombinant protein?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the advantages of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the limitations of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the advantages of the bacterial protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What is the limitation of the bacteria protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the advantages of the insect protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the limitations of the insect protein expression system?
BioconjugationAntibody and Protein LabelingDNA and RNAProtein


Posted on August 5, 2023
What are the advantages of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the limitations of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the applications of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the applications of the bacterial protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the applications of the insect protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the applications of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the applications of cell-free protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What is the principle of cell-free protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What factors should I consider when choosing protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the pros of the baculovirus expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
When does protein expression occur?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on August 5, 2023
What are the types of extracts used for cell-free protein expression?
Antibodies and Proteomics BioconjugationDNA and RNAProtein


Posted on July 24, 2023
What are the precautions or limitations when using propidium iodide?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
Can propidium iodide be used in live cell imaging?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
What are the applications of propidium iodide?
Apoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
How is propidium iodide used in flow cytometry?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 12, 2023
What would happen if the nucleus had no membrane?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
How does the nucleus control gene expression?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Can the nucleus be found in all types of cells?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What happens to the nucleus during cell division?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What are the main functions of the nucleus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
How does the nucleus communicate with the cytoplasm?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Can the nucleus undergo division?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Can the nucleus be affected by diseases?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
How does the nucleus contribute to cell division?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Can substances move in and out of the nucleus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Can mutations occur in the nucleus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Are all cell nuclei in the body the same?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What is the significance of the nucleus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What are the common shapes of the nucleus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What is the structure of the nucleolus?
DNA and RNANucleic AcidsNucleus


Posted on July 12, 2023
What are the functions of nucleolus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
What are the differences between nucleus and nucleolus?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 12, 2023
Where is the nucleolus located in the cell?
BioconjugationCellular Structures and OrganellesDNA and RNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on July 10, 2023
What is the significance of synthesis of aminoacyl-tRNAs?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAProteinamino acid sequence


Posted on July 10, 2023
What are the steps of the synthesis of aminoacyl-tRNAs
ATPCell SignalingCellular ProcessesDNA and RNAEnzymesProteinamino acid sequence


Posted on July 10, 2023
How does protein synthesis occur in eukaryotes?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Protein


Posted on July 10, 2023
What is the role of nucleotides in protein synthesis?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAProteinamino acid sequence


Posted on May 20, 2023
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on May 20, 2023
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 19, 2023
What are the applications of proteinase K?
Cellular ProcessesDNA and RNAEnzymesPolymerase Chain Reaction (PCR)Protein


Posted on May 19, 2023
What are the types of enzymes?
Cell Cycle and ProliferationCell MetabolismCellular ProcessesDNA and RNAEnergyEnzymesTransferases


Posted on May 12, 2023
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing


Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 2, 2023
What is the significance of protein and DNA labeling?
Antibodies and ProteomicsAntibody and Protein LabelingDNA and RNANucleic AcidsNucleus


Posted on May 2, 2023
What are features of next generation sequencing?
DNADNA and RNADNA sequencingNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
What are the limitations of Sanger sequencing?
DNADNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
Which technique is used to visualize DNA following a PCR reaction?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Proteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How do DNA fragments move through the gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What factors should I consider before I use restriction enzymes to cut DNA into small fragments?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on May 2, 2023
How does the cell protect its own DNA from disassembly?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleus


Posted on May 2, 2023
What is the significance of running an uncut DNA on electrophoresis gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
When should I use 1kb DNA ladder?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleuspolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder visualized in gel electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder used in gel electrophoresis?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRpolyacrylamide geldna ladder


Posted on May 2, 2023
What are the limitations of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsProteinnucleic acidpolyacrylamide gel


Posted on May 2, 2023
What are the limitations of in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What should be considered when I do in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of in-gel staining?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gelprotein


Posted on April 27, 2023
What are the differences between mRNA and tRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents


Posted on April 27, 2023
What are the similarities between mRNA and tRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents


Posted on April 27, 2023
What are the functions of mRNA?
Antibodies and ProteomicsDNA and RNANucleusRNATransfectionTransfection Reagents


Posted on April 27, 2023
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Antibodies and ProteomicsDNA and RNANucleusRNATransfectionTransfection Reagents


Posted on April 27, 2023
What is the structure of mRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents


Posted on April 27, 2023
What happens to mRNA after processing is complete?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents


Posted on April 27, 2023
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium


Posted on April 27, 2023
What happens if I add too little MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAEnzymesPCR primerPolymerase Chain Reaction (PCR)


Posted on April 27, 2023
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on April 19, 2023
What is the mechanism of exonuclease?
Cellular ProcessesCellular Structures and OrganellesDNA and RNADNA and RNA QuantitationEnzymesNucleic Acids


Posted on April 19, 2023
What is the mechanism of endonuclease?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleic Acids


Posted on April 19, 2023
How does a promoter affect DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleic Acids


Posted on April 19, 2023
What is the role of a promoter?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic Acids


Posted on April 19, 2023
What are the similarities between DNA and cDNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between DNA and cDNA?
Cellular ProcessesCellular Structures and OrganellesDNA and RNADNA and RNA QuantitationNucleic AcidsNucleus


Posted on April 19, 2023
What is the basic structure of a gene?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusTransfectionTransfection Reagentsgene sequencing


Posted on April 19, 2023
Where are alleles located?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the functions of genes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusgene sequencing


Posted on April 19, 2023
What are the functions of alleles?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between genes and alleles?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between gene and DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
Is junk DNA completely useless?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What does DNA do?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between the sugar in DNA and the sugar in RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on March 27, 2023
Which cell organelle is called the headquarter of the cell and why?
Cellular ProcessesCellular Structures and OrganellesDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on March 1, 2023
What is the end product of transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the differences between cDNA and genomic DNA library?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the significance of cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the workflow of cDNA library construction?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the advantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the disadvantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the steps to construct a genomic DNA library?
DNA and RNA QuantitationDNA and RNAEnzymesGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the applications of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on March 1, 2023
Where does the transcription terminate?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
Are enhancers necessary for transcription?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the promoter sequences?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor


Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 24, 2023
Where does protein synthesis occur?
DNA and RNAProteinRNA


Posted on February 24, 2023
What are the differences between Protein Synthesis in Prokaryotes and Eukaryotes?
DNA and RNANucleic AcidsNucleusProteinRNA


Posted on February 24, 2023
What does ATP do in protein synthesis?
ATPDNA and RNANucleic AcidsNucleusProteinRNA


Posted on February 24, 2023
What are the steps of protein synthesis?
Cellular ProcessesDNA and RNANucleic AcidsNucleusProteinRNA


Posted on February 24, 2023
What methods can I use to measure and detect translation?
DNA and RNANucleic AcidsNucleusProteinRNA


Posted on February 24, 2023
Where does amino acid activation occur during protein synthesis?
Cellular ProcessesDNA and RNAProteinRNA


Posted on February 24, 2023
What enzyme is involved in amino acid activation?
DNA and RNAEnzymesNucleic AcidsNucleusProteinRNA


Posted on February 24, 2023
What is the mechanism of rRNA charging?
DNA and RNANucleic AcidsNucleusProteinRNA


Posted on February 23, 2023
What are the similarities between CDS and cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 23, 2023
Where is reverse transcriptase found?
DNA and RNA QuantitationDNA and RNAE. coliEnzymesbacteria


Posted on February 23, 2023
What are the differences between CDS and cDNA?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
How do I control and regulate gene expression?
DNA and RNA QuantitationDNA and RNA


Posted on February 23, 2023
What are the applications for reverse transcriptase?
DNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What is the function of reverse transcriptase?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
How does reverse transcriptase work?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What are the different technologies available to analyze transcription factors?
DNA and RNA QuantitationDNA and RNAWestern Blot


Posted on February 23, 2023
What are the methods used to measure and detect transcription?
DNA and RNA QuantitationDNA and RNANucleusRNAReal-Time PCR (qPCR)in situ hybridizationqPCR


Posted on February 23, 2023
What are the major enzymes used in DNA transcription?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleusPolymerases


Posted on February 22, 2023
What are the similarities in the transcription between eukaryotes and prokaryotes?
Cell Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the differences in the transcription between eukaryotes and prokaryotes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What is the function of promoters?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the functions of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the types of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 22, 2023
What is the structure of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 22, 2023
How do transcription factors work?
Cellular Structures and OrganellesDNA and RNADNA and RNA QuantitationNucleic AcidsNucleus


Posted on February 22, 2023
How can I tell whether a regulator protein is an activator or an inhibitor?
Cellular Structures and OrganellesDNADNA and RNANucleic AcidsNucleus


Posted on February 22, 2023
Where are genes transcribed?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleuscytoplasm


Posted on February 22, 2023
Are all transcribed genes translated into proteins?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusProtein


Posted on February 20, 2023
How does mRNA, tRNA and rRNA work together?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What are the two different types of rRNAs?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where does the rRNA function in cells?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where is rRNA located?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What is the basic structure of RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What is the structural difference between ribose and deoxyribose?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where is mRNA synthesized?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
How does mRNA work?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What happens during the synthesis of mRNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 17, 2023
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on February 17, 2023
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
How do I remove the RNA primers?
DNA and RNAEnzymesPolymerase Chain Reaction (PCR)Polymerases


Posted on February 17, 2023
What happens if I only use a forward primer?
DNA and RNAPCR primerPolymerase Chain Reaction (PCR)


Posted on February 14, 2023
What happens if DNA replication does not occur?
Cell Cycle AnalysisCell Cycle AssaysDNA and RNA QuantitationDNA and RNA


Posted on February 14, 2023
What is the process of DNA replication?
Cell Cycle AnalysisCell Cycle AssaysDNA and RNA QuantitationDNA and RNAPolymerases


Posted on February 14, 2023
How many types of RNA are formed during transcription?
DNA and RNA QuantitationDNA and RNARNA


Posted on February 14, 2023
What are the similarities between endonuclease and exonuclease?
Cellular Structures and OrganellesDNA and RNAEnzymesNucleic AcidsNucleus


Posted on February 13, 2023
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What are the advantages of PCR (polymerase chain reaction)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What are the common PCR additives?
BSADMSODNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)bovine serum albumindetergentmagnesium


Posted on February 13, 2023
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How do I find the concentration of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What primer concentration should I use for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)


Posted on February 10, 2023
What are the functions of nuclear pores?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
Where are nuclear pores found?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
What are the differences between nuclear membrane and nuclear envelope?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
What are the similarities between nuclear membrane and nuclear envelope?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
What is the structure of the nuclear pore complex?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
What is the nuclear membrane made of?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 10, 2023
What is the structure of a nuclear membrane?
Cellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on February 9, 2023
How do bacteria reproduce?
BioconjugationCellular Structures and OrganellesDNA and RNAbacteria


Posted on February 9, 2023
How many different types of RNA polymerases are involved in synthesis of all rRNA of 80s ribosomes?
Cellular Structures and OrganellesDNA and RNAEnzymesPolymerasesRNA


Posted on February 9, 2023
Do bacterial cells have DNA?
BioconjugationCellular Structures and OrganellesDNA and RNA


Posted on February 9, 2023
What are the differences between bacteria and eukaryotes?
BioconjugationCellular Structures and OrganellesDNA and RNAbacteria


Posted on February 7, 2023
What are the main factors that can affect DNA stability in solution?
Cellular Structures and OrganellesDNA and RNANucleus


Posted on February 7, 2023
What are the differences between mitochondrial dna and chloroplast dna?
Cellular Structures and OrganellesDNA and RNANucleus


Posted on February 7, 2023
What are the similarities between mitochondrial DNA and chloroplast dna?
Cellular Structures and OrganellesDNA and RNANucleus


Posted on February 7, 2023
What are the characteristics of DNA structure?
Cellular Structures and OrganellesDNA and RNANucleus


Posted on February 7, 2023
What are the similarities between DNA methylation and histone acetylation?
Antibodies and ProteomicsCellular Structures and OrganellesDNA and RNANucleus


Posted on February 7, 2023
What are the differences between DNA methylation and histone acetylation?
Antibodies and ProteomicsCellular Structures and OrganellesDNA and RNANucleus


Posted on January 26, 2023
Why are dead cells problematic in flow cytometry?
Antibodies and Proteomics BioconjugationCell Viability AssaysCellular ProcessesDNA and RNALive or DeadSpectral Flow Cytometry


Posted on January 26, 2023
How does the fixed cell viability staining dyes work?
Cell Viability AssaysCellular ProcessesDNA and RNAFlow Cytometry ReagentsLive or DeadProteinfixable


Posted on January 26, 2023
How are viruses and cells similar?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymes


Posted on January 26, 2023
What are the similarities between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein


Posted on January 26, 2023
What are the differences between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNANucleic Acids


Posted on January 26, 2023
What are the types of viruses based on the type of host?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesbacteria


Posted on January 26, 2023
Are viruses considered living?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein


Posted on January 26, 2023
What are the basic components of virions?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic AcidsNucleusProtein


Posted on January 26, 2023
How are viruses classified?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic Acidsbacteria


Posted on January 26, 2023
What are the steps of viral replication?
Antibodies and Proteomics BioconjugationCellular ProcessesDNA and RNAEnzymesNucleic AcidsProteinbacteria


Posted on January 26, 2023
What are the differences between lytic and lysogenic?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProtein


Posted on January 26, 2023
What happens in the lysogenic cycle?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProteinbacteria


Posted on January 26, 2023
What happens during lysogenic conversion?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProteinbacteria


Posted on January 26, 2023
What are the differences between DNA viruses and RNA viruses?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsNucleusProtein


Posted on January 26, 2023
What is Baltimore classification?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProtein


Posted on January 23, 2023
What are the types of DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
How do acetylation and phosphorylation affect DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What is a histone made of?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What are the major functions of histones in the cell?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What role do histones play in DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What is the importance of DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
How is DNA packaged in a cell?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What are the similarities between promoters and enhancers?
DNA and RNA QuantitationDNA and RNADNA sequencingPolymerase Chain Reaction (PCR)


Posted on January 20, 2023
What are the similarities between plasmid DNA and genomic DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the functions of plasmids?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the common types of plasmids?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the applications of plasmids?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the properties of a plasmid?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What should I do if I get very few, if any, transformants when transforming large plasmids ((>10 kb) or BACS?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the methods for plasmid detection?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the important features required for a plasmid vector?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
How do I increase plasmid yield?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNA


Posted on January 20, 2023
How is plasmid removed from DNA?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 18, 2023
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on January 18, 2023
What are the steps of emulsion PCR?
DNA and RNA QuantitationDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 18, 2023
How can I avoid primer-dimer formation during PCR amplification?
DNA and RNA QuantitationDNA and RNAPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 6, 2023
What are the differences between eukaryotic transcription and prokaryotic transcription?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on September 16, 2022
Do all cells use ATP?
ATPCell Cycle and ProliferationCell Proliferation AssaysCellular MetabolismCellular ProcessesDNA and RNAEnergy


Posted on August 18, 2022
What are the processing steps for messenger RNAs?
BioconjugationDNA and RNADNA and RNA QuantitationTransfection Reagents


Posted on October 28, 2021
What does RNA polymerase do in bacteria?
EnzymesDNA and RNAPhysiological Probes


Posted on October 25, 2021
Does RNA polymerase need a primer?
Reverse Transcription PCR (RT-PCR)DNA and RNANucleusPhysiological Probes


Posted on October 25, 2021
Does RNA polymerase make RNA?
DNA and RNANucleusPhysiological ProbesMembrane Potential and Channels


Posted on October 25, 2021
How does RNA polymerase work?
DNA and RNANucleusPhysiological Probes


Posted on May 7, 2021
How does Hoechst work?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
Does Hoechst 33342 stain dead cells?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
What is the best concentration to use with Hoechst?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
Do I need to permeabilize cells for Hoechst staining?
NuleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
Is Hoechst toxic to cells?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
Can Hoechst be used to stain bacteria?
NucleusPhysiological ProbesDNA and RNA


Posted on May 6, 2021
Can Hoechst stain live cells?
Cell Structures and OrganellesNucleusDNA and RNA


Posted on May 6, 2021
What part of the DNA strand does Hoechst bind to?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
What kind of excitation light does Hoechst require?
NucleusDNA and RNABioconjugation


Posted on May 6, 2021
How does Hoechst bind to DNA?
NucleusDNA and RNA


Posted on May 6, 2021
Is Hoechst cell permeable?
NucleusDNA and RNA


Posted on May 6, 2021
What is the role of Hoechst DNA staining in the diagnosis of mycoplasma infection?
NucleusDNA and RNAPhysiological Probes


Posted on May 6, 2021
What interaction makes Hoechst fluorescence blue?
NucleusDNA and RNA


Posted on May 5, 2021
Is Hoechst cytotoxic?
NucleusDNA and RNABioconjugation


Posted on May 5, 2021
What does Hoechst stain?
NucleusDNA and RNABioconjugation


Posted on May 3, 2021
What are the applications of CRISPR?
DNA and RNA


Posted on May 3, 2021
How does CRISPR work?
DNA and RNA


Posted on April 19, 2021
What makes up the chromosomes of cells?
DNA and RNAGel ElectrophoresisNucleus


Posted on April 9, 2021
What happens after the acetylation of lysines?
Chemical ReagentsDNA and RNA


Posted on April 9, 2021
Are methyl groups positive or negative?
DNA and RNAPhysiological Probes


Posted on March 25, 2021
What are the effects of mutations?
DNA and RNAPhysiological Probes


Posted on March 25, 2021
Are all mutations harmful?
DNA and RNAPhysiological Probes


Posted on March 25, 2021
What are the major types of mutations?
DNA and RNAPhysiological ProbesNucleus


Posted on March 25, 2021
What causes mutations?
NucleusDNA and RNAPhysiological Probes


Posted on March 25, 2021
How is the Ames test performed?
NucleusDNA and RNAPhysiological Probes


Posted on March 25, 2021
Why is salmonella used in the Ames test?
DNA and RNANucleusPhysiological Probes


Posted on March 25, 2021
What is the principle of the Ames test?
DNA and RNANucleusPhysiological Probes


Posted on March 24, 2021
What are the applications of the Ames test?
DNA and RNAEnzymes


Posted on March 24, 2021
Are substances that test positive with the Ames test necessarily carcinogenic in humans?
DNA and RNAEnzymes


Posted on March 22, 2021
What are the advantages of the Ames test for mutation detection?
EnzymesDNA and RNANucleus


Posted on March 22, 2021
What limitations does the Ames test have?
EnzymesDNA and RNANucleus


Posted on March 22, 2021
What is the Ames test used for?
EnzymesDNA and RNANucleus


Posted on February 18, 2021
How many nucleotides is RNA composed of?
NucleusDNA and RNAPhysiological Probes


Posted on February 11, 2021
What is the difference between G1 and S phase in the cell cycle?
DNA and RNABioconjugationPhysiological ProbesNucleus


Posted on February 11, 2021
When does DNA replication take place?
DNA and RNAPhysiological ProbesNucleus


Posted on February 11, 2021
What are the reasons of unusually high extent of cells in the S-phase?
DNA and RNAPhysiological ProbesNucleus


Posted on February 11, 2021
How much BSA should I add to PCR?
DNA and RNAPhysiological ProbesBioconjugation


Posted on February 8, 2021
What is RNA stability?
DNA and RNAphysiological Probes


Posted on February 8, 2021
How long is RNA stable at room temperature?
DNA and RNAPhysiological Probes


Posted on February 5, 2021
Is RNA less stable than DNA?
DNA and RNAPhysiological Probes


Posted on February 4, 2021
What is a bacteriophage?
EnzymesDNA and RNANuleus


Posted on February 4, 2021
Why does DNA not form protein directly?
Physiological ProbesDNA and RNABioconjugation


Posted on February 4, 2021
Is DNA a protein?
DNA and RNAPhysiological Probes


Posted on February 3, 2021
What are the differences between prokaryotes and eukaryotes?
BioconjugationDNA and RNA


Posted on February 3, 2021
What is a TATA box?
DNA and RNAPhysiological Probes


Posted on February 3, 2021
What is the difference between miRNA and mRNA?
DNA and RNAPhysiological Probes


Posted on February 3, 2021
When are restriction enzymes used?
DNA and RNANucleusPhysiological Probes


Posted on January 28, 2021
What are the most common types of nucleic acids?
NucleusDNA and RNAPhysiological Probes


Posted on January 27, 2021
What is the difference between endocytosis and exocytosis?
Intracellular pHDNA and RNA


Posted on January 26, 2021
What is the pKa of the phosphate group in DNA and RNA molecules?
DNA and RNACell SignalingPhysiological ProbesCellular Processes


Posted on January 26, 2021
What is the pH of DNA?
DNA and RNANucleusPhysiologicalBioconjugation


Posted on January 26, 2021
What are three types of RNA?
NucleusDNA and RNACellular ProcessesCell Cycle and Proliferation


Posted on January 26, 2021
What are the difference between DNA and RNA?
DNA and RNAPhysiological ProbesNucleus


Posted on January 25, 2021
At what pH does DNA denature?
Cell MetabolismEnzymesDNA and RNA


Posted on January 25, 2021
How does pH affect DNA stability?
Cell MetabolismEnzymesDNA and RNA


Posted on January 22, 2021
How is DNA methylation detected?
DNA and RNAPhysiological ProbesNucleus


Posted on January 8, 2021
What is the relationship between DNA, RNA and proteins?
NucleusDNA and RNABioconjugationPhysiological Probes


Posted on January 8, 2021
What's best for DNA quantification, gel electrophoresis or NanoDrop?
DNA and RNANucleusPhysiological Probes


Posted on December 16, 2020
What is qPCR?
DNA and RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on December 8, 2020
At what temperature does RNA denature?
NucleusDNA and RNAPhysiological Probes


Posted on December 8, 2020
What is the best lysis buffer for protein extraction?
DNA and RNAPhysiological ProbesNucleusTools


Posted on December 7, 2020
What's the difference between an enhancer and a promoter?
DNA and RNA


Posted on December 5, 2020
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 30, 2020
How do I calculate PCR efficiency?
DNA and RNAChemical ReagentsClassic Dyes


Posted on November 30, 2020
What is the acceptable PCR primer efficiency value?
DNA and RNAPhysiological ProbesNucleusChemical Reagents


Posted on November 25, 2020
What affects PCR efficiency?
NucleusDNA and RNAPhysiological Probes


Posted on November 25, 2020
What is stringency in hybridization?
Chemical ReagentsPhysiological ProbesDNA and RNANucleus


Posted on November 25, 2020
What are the common methods for nucleic acid labeling?
Physiological ProbesNucleusDNA and RNABioconjugation


Posted on November 25, 2020
How do I evaluate the performance of a real-time PCR reaction?
Classic DyesDNA and RNAChemical Reagents


Posted on November 25, 2020
Why do I get an unexpected band or multiple bands for my PCR result?
DNA and RNANulecusPhysiological Probes


Posted on November 24, 2020
Is qPCR efficiency under 90% acceptable?
NucleusDNA and RNAPhysiological ProbesCell Structures and Organelles


Posted on November 24, 2020
How do I increase the intensity of PCR bands?
DNA and RNANucleusPhysiological Probes


Posted on November 24, 2020
Why I'm getting amplification even without template in PCR reaction?
DNA and RNAPhysiological ProbesNucleus


Posted on November 24, 2020
How can I improve the PCR output of a low concentrated DNA?
Chemical ReagentsDNA and RNAPhysiological Probes


Posted on November 23, 2020
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 19, 2020
What is a good RNA concentration?
NucleusDNA and RNAPhysiological Probes


Posted on November 19, 2020
What are the differences between Northern blot, Southern blot and Western blot?
Protein to Protein ConjugationDNA and RNANucleus


Posted on November 4, 2020
What is blunt end ligation?
NucleusDNA and RNAPhysiological Probes


Posted on November 4, 2020
What is Golden Gate assembly?
DNA and RNA


Posted on November 3, 2020
What are the three stop codons?
DNA and RNAPhysiological Probes


Posted on October 29, 2020
What is the function of RNA?
DNA and RNANucleusPhysiological Probes


Posted on October 21, 2020
What are the difference between an operator and a promoter?
DNA and RNA


Posted on October 21, 2020
What is a promoter in biology?
DNA and RNA


Posted on October 21, 2020

DNA and RNACell Structures and OrganellesPhysiological Probes


Posted on October 21, 2020
What are the differences between chromatin and chromosome?
Cell Structures and OrganellesDNA and RNACellular Processes


Posted on October 19, 2020
Why does my DNA oligonucleotide have mutations?
Peptide and Oligonucleotide LabelingDNADNA and RNAPeptide and Oligonucleotide


Posted on September 30, 2020
Why is EDTA used in lysis buffer?
DNA and RNACell Structures and OrganellesPhysiological Probes


Posted on September 30, 2020
What is the best DNA extraction method for genomics applications?
DNADNA and RNA QuantitationDNA and RNADNA extractionPolymerase Chain Reaction (PCR)


Posted on September 30, 2020
How do I lyse cells?
Cell Cycle AnalysisCellular Structures and OrganellesDNA and RNAbuffercell lysis


Posted on September 29, 2020
How can I know if a plasmid DNA is pure?
DNA and RNAPhysiological Probes


Posted on September 28, 2020
How do I isolate plasmid DNA from E coli?
DNADNA and RNAPhysiological Probes


Posted on September 25, 2020
What is the function of primers in DNA PCR process?
NucleusDNA and RNAPhysiological Probes


Posted on September 25, 2020
How do I remove genomic DNA contamination from isolated plasmid DNA?
DNADNA and RNA QuantitationDNA PurityDNA and RNA


Posted on July 22, 2020
What methods are used in next-generation sequencing (NGS) to amplify templates?
DNADNA and RNADNA sequencingPolymerase Chain Reaction (PCR)gene sequencing


Posted on July 22, 2020
How does bridge amplification work in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by synthesis approach in next-generation sequencing (NGS)?
Cellular processesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by ligation approach in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is a reversible terminator used in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What types of reversible terminators are available for next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the major platforms for next generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is the general procedure of Illumina sequencing?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is pyrosequencing?
DNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does pyrosequencing work?
DNADNA and RNA QuantitationDNA and RNADNA sequencingEnzymesgene sequencing


Posted on July 22, 2020
What is third-generation sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What methods are commonly used to label the probes in fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePeptide and OligonucleotidePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 22, 2020
What is a restriction enzyme?
Cell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesDNA and RNAEnzymesProtein


Posted on July 22, 2020
What are sticky and blunt ends of DNA molecules?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
How many restriction enzymes are used to insert a specific DNA sequence into a vector in molecular cloning?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is the difference between endonuclease and exonuclease?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is the role of exonuclease in DNA replication?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
Does DNA polymerase I have exonuclease activity?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is a replication fork?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is a leading strand and a lagging strand?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is the role of primase in DNA replication?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is the asymmetry of DNA replication?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is DNA ligase? And what is it used for?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is the enzymatic mechanism of ligase?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
Does the leading strand require DNA ligase?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
What is ligase chain reaction?
Cellular ProcessesDNA and RNAEnzymesPhysiological Probes


Posted on July 22, 2020
How do you design sgRNA sequences for CRISPR-Cas9?
DNADNA and RNAEnzymesRNAbacteriadna sequencing


Posted on July 22, 2020
What is difference between CRISPR interference and CRISPR activation?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on July 22, 2020
What is the difference between CRISPRi and RNAi?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on July 22, 2020
How does siRNA work in RNA interference (RNAi)?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on July 22, 2020
What are the differences between miRNA and siRNA?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What are the different kinds of RNAs and what do they do?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is gene silencing?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is the difference between gene silencing and gene knockout?
DNADNA and RNANucleic AcidsRNAgene sequencing


Posted on July 22, 2020
How do antisense oligonucleotides work in gene silencing?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideRNAgene sequencing


Posted on July 22, 2020
What are the types of DNA repair mechanisms?
DNADNA and RNANucleic AcidsRNAdsDNAssDNA


Posted on July 22, 2020
What is the difference between DNA damage and DNA mutation?
DNADNA and RNADNA sequencingRNAdsDNAssDNA


Posted on July 22, 2020
What is the role of p53 in DNA damage repair system?
Cell Cycle AnalysisDNADNA and RNAProteinRNA


Posted on July 22, 2020
What is fluorescence in situ hybridization (FISH)?
DNADNA and RNAFISHFluorescenceNucleic AcidsRNAfluorescence microscopein situ hybridization


Posted on July 22, 2020
What are the general steps of fluorescence in situ hybridization (FISH) experiments?
DNADNA and RNAFISHFluorescencePolymerase Chain Reaction (PCR)RNAin situ hybridizationssDNA


Posted on July 22, 2020
What is nick translation?
DNADNA and RNADNA sequencingFISHPeptide and Oligonucleotidein situ hybridization


Posted on July 22, 2020
What types of probes are used in fluorescence in situ hybridization (FISH)?
DNA and RNAFISHFluorescencein situ hybridization


Posted on July 22, 2020
How do you prepare probes for fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 15, 2020
6-ROX is predominately used as a reference dye for performing PCR detections, however, 6-ROX is very unstable compared to other rhodamine dyes. What is a better dye replacement?
Chemical ReagentsDNA and RNADNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the protospacer adjacent motif (PAM) in CRISPR-Cas9?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How is Cas prevented from cutting its own CRISPR locus?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How does CRISPR-Cas9 edit genes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What are the two main types of DNA repair mechanisms employed in CRISPR-Cas9?
DNADNA and RNAEnzymesbacteriadna sequencing


Posted on June 22, 2020
How is CRISPR-Cas9 used in gene knockout and knock-in?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is dCas9? And what is its application?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is the difference between transcription and reverse transcription?
DNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What are introns?
DNADNA and RNADNA sequencingNucleic AcidsProteinRNAgene sequencing


Posted on June 22, 2020
What are the types of introns?
Antibodies and ProteomicsDNADNA and RNAProteinRNA


Posted on June 22, 2020
What are the functions of introns?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is RNA splicing?
DNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What are the three major steps in mRNA processing?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is the function of the 5’ cap and 3’ poly-A tail in mRNA?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What are exons?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is translation in biology?
Cellular ProcessesDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is needed for translation?
Cellular ProcessesDNADNA and RNAProteinRNA


Posted on June 22, 2020
What is the function of ribosome?
Cellular ProcessesDNADNA and RNANucleic AcidsProteinRNA


Posted on June 22, 2020
What is a codon?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What are the three stages of translation?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What are the 3 binding sites for tRNA in the ribosome?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNACell Structure and Organelles


Posted on June 22, 2020
What happens to mRNA after it gets translated?
Cellular ProcessesDNADNA and RNANucleic AcidsProteinRNA


Posted on June 22, 2020
What is exon shuffling?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on June 22, 2020
What is genetic recombination?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on June 22, 2020
What is recombinant DNA?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is molecular cloning?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between PCR and molecular cloning regarding DNA replication?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What are the main steps of cloning?
DNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)Purification


Posted on June 22, 2020
What is a cloning vector in molecular biology?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the essential features of a plasmid cloning vector?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
Why are plasmids used as vectors in genetic engineering?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is transformation in molecular biology?
Cellular ProcessesDNADNA and RNANucleic AcidsTransfectionTransformation


Posted on June 22, 2020
What is horizontal gene transfer?
Cellular ProcessesConjugationDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Transformation


Posted on June 22, 2020
What is cloned DNA used for?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between cloning and expression vectors?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the features of mammalian expression system?
DNA and RNAProtein


Posted on June 22, 2020
What is CRISPR?
DNADNA and RNADNA sequencingNucleic Acidsbacteria


Posted on June 22, 2020
What is the CRISPR-Cas system in prokaryotes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What are the three stages in the CRISPR-Cas defense system?
DNADNA and RNAEnzymesbacteriadna sequencing


Posted on June 22, 2020
What is CRISPR-Cas9 technology?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is a single guide RNA?
DNADNA and RNADNA sequencingEnzymesRNA


Posted on June 22, 2020
What are the main components of CRISPR-Cas9 technique?
DNADNA and RNAEnzymesRNAbacteria


Posted on June 22, 2020
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
What is a sense strand (or coding strand)?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)dsDNAssDNA


Posted on June 22, 2020
How do you design a fluorescent hydrolysis probe in qPCR?
DNA and RNA QuantitationDNA and RNAReal-Time PCR (qPCR)qPCR


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 1, 2020
What is gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
What is the function of electrophoresis buffer?
DNA and RNA QuantitationDNA and RNAGel Electrophoresisbuffer


Posted on June 1, 2020
What is the difference between denaturing and non-denaturing (native) gels?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisNucleic AcidsPhysiological Probes


Posted on June 1, 2020
What affects the migration rate of nucleic acid in gel?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPhysiological Probesethidium bromide


Posted on June 1, 2020
Why aren’t SDS-PAGE gels horizontal?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresis


Posted on May 18, 2020
How is RNA isolated?
Antibodies and ProteomicsDNA and RNAExtractionFlow Cytometry ReagentsPhysiological ProbesPurificationRNASpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is the function of chloroform in RNA extraction?
Chemical ReagentsCellular Structures and OrganellesDNA and RNAFlow Cytometry ReagentsPurificationRNASpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
Why is RNA extraction tougher than DNA extraction?
Cellular Structures and OrganellesDNADNA and RNADNA extractionExtractionRNA


Posted on May 18, 2020
How do you evaluate the transfection efficiency?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 18, 2020
What is the difference between transient and stable transfection?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 18, 2020
What transfection methods are commonly used in biology?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 9, 2020
What is next-generation sequencing (NGS)?
Chemical ReagentsDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Can next-generation sequencing (NGS) sample preparation be automated?
DNA and RNADNA sequencingPhysiological Probesgene sequencing


Posted on May 9, 2020
What is a next-generation sequencing (NGS) library?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencingqPCR


Posted on May 9, 2020
How pure does DNA have to be for next-generation sequencing (NGS)?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
What is targeted next-generation sequencing (NGS)?
DNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)dna sequencing


Posted on May 9, 2020
How long does next-generation sequencing (NGS) take?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the difference between next-generation (NGS) and high-throughput sequencing (HTS)?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Is next-generation sequencing (NGS) better than Sanger sequencing?
DNA and RNA QuantitationDNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Can next-generation sequencing (NGS) be used for virus genomes?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)RNA


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
What is the difference between nuclease-free water and ultrapure water?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic Acids


Posted on May 9, 2020
What are oligonucleotides?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNAFRETPeptide and Oligonucleotidefluorescence imaging


Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR


Posted on May 9, 2020
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer


Posted on April 24, 2020
What is the difference between recombinant and synthetic polypeptides?
Antibodies and Proteomics BioconjugationDNA and RNAPeptide and OligonucleotideProtein


Posted on April 24, 2020
What is the difference between 'operon' and 'transcription unit'?
Cellular ProcessesCellular Structures and OrganellesDNADNA and RNAEnzymesNucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on April 24, 2020
What is the major difference between Northern and Southern blotting?
Antibodies and ProteomicsAgarose gel electrophoresisDNA and RNAEnzymesGel ElectrophoresisNucleic AcidsProteinWestern Blotpolyacrylamide gel


Posted on April 24, 2020
What is the difference between a serotype and a subtype of a virus?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDNA and RNAEnzymesProtein


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 24, 2020
What is the difference between ethidium homodimer and dihydroethidium in terms of staining?
Peptide and Oligonucleotide LabelingCell ViabilityDNA and RNA QuantitationDNA and RNAFluorescenceNucleic AcidsPhysiological Probes


Posted on April 17, 2020
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer


Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization


Posted on April 17, 2020
How does transfection with lipofectamine 2000 work?
LipofectamineLipofectamine 2000TransfectionTransfection ReagentsDNA and RNANucleic Acids


Posted on April 17, 2020
What are the differences between stable and permanent transfection/transduction?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic AcidsPhysiological ProbesTransfectionTransfection Reagents


Posted on April 17, 2020
What is the purpose of a buffered solution used for RNase treatment?
Chemical ReagentsBuffer Preparations and RecipesDNA PurityDNA and RNAEnzymesNucleic Acidsbuffer


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 4, 2020
What is the significance of DNA quantification?
DNA quantificationDNA and RNA